ETHIDIUM BROMIDE

MSDS Number: E2410 --- Effective Date: 12/08/96


1. Product Identification

Synonyms: Homidium bromide; dromilac; 3,8-diamino-5-ethyl-6-phenyl phenanthridinium bromide
CAS No.: 1239-45-8
Molecular Weight: 394.31
Chemical Formula: C21H20BrN3
Product Codes: J.T. Baker: 4007 Mallinckrodt: 7738


2. Composition/Information on Ingredients


  Ingredient                                CAS No         Percent   Hazardous                                       
  ---------------------------------------   ------------   -------   ---------        
 
  Ethidium Bromide                          1239-45-8      90 - 100%    Yes                                                                  
 


3. Hazards Identification

Emergency Overview
--------------------------
WARNING! MAY BE HARMFUL IF SWALLOWED, INHALED OR ABSORBED THROUGH SKIN. MAY CAUSE IRRITATION TO SKIN, EYES, AND RESPIRATORY TRACT.


J.T. Baker SAF-T-DATA(tm) Ratings (Provided here for your convenience)
-----------------------------------------------------------------------------------------------------------
Health Rating: 3 - Severe (Life)
Flammability Rating: 1 - Slight
Reactivity Rating: 1 - Slight
Contact Rating: 3 - Severe (Life)
Lab Protective Equip: GOGGLES; LAB COAT; VENT HOOD; PROPER GLOVES
Storage Color Code: Orange (General Storage)
-----------------------------------------------------------------------------------------------------------

Potential Health Effects
----------------------------------

No specific health hazard information on this compound was found. However, its chemical structure and those of other polycyclica suggest that the following warnings are appropriate.

Inhalation:
Moderate irritant. May cause coughing or sneezing.

Ingestion:
The material should be assumed to be at least moderately hazardous. Gastrointestinal upset with possible liver or kidney damage are suggested by the compound's structure.

Skin Contact:
The material should be assumed to be a moderate irritant as well as a dye. Inflammation and discoloration of the skin may occur after contact.

Eye Contact:
Possible irritant. Ethidium bromide may be absorbed through eye tissues with unknown results. See Ingestion, above.

Chronic Exposure:
No information found.

Aggravation of Pre-existing Conditions:
No information found.


4. First Aid Measures

Inhalation:
Remove to fresh air. Get medical attention for any breathing difficulty.

Ingestion:
Induce vomiting immediately as directed by medical personnel. Never give anything by mouth to an unconscious person. Get medical attention.

Skin Contact:
Immediately flush skin with plenty of water for at least 15 minutes. Remove contaminated clothing and shoes. Wash clothing before reuse. Thoroughly clean shoes before reuse. Get medical attention if irritation develops.

Eye Contact:
Immediately flush eyes with plenty of water for at least 15 minutes, lifting upper and lower eyelids occasionally. Get medical attention if irritation persists.


5. Fire Fighting Measures

Fire:
As with most organic solids, fire is possible at elevated temperatures or by contact with an ignition source.

Explosion:
Not an explosion hazard under normal conditions of storage and use, but fine dust dispersed in air in sufficient concentrations, and in the presence of an ignition source is a potential dust explosion hazard. Sensitive to static discharge.

Fire Extinguishing Media:
Water spray, dry chemical, alcohol foam, or carbon dioxide.

Special Information:
In the event of a fire, wear full protective clothing and NIOSH-approved self-contained breathing apparatus with full facepiece operated in the pressure demand or other positive pressure mode.


6. Accidental Release Measures

Remove all sources of ignition. Ventilate area of leak or spill. Wear appropriate personal protective equipment as specified in Section 8. Spills: Clean up spills in a manner that does not disperse dust into the air. Use non-sparking tools and equipment. Reduce airborne dust and prevent scattering by moistening with water. Pick up spill for recovery or disposal and place in a closed container.


7. Handling and Storage

Avoid dust formation and control ignition sources. Employ grounding, venting and explosion relief provisions in accord with accepted engineering practices in any process capable of generating dust and/or static electricity. Empty only into inert or non-flammable atmosphere. Emptying contents into a non-inert atmosphere where flammable vapors may be present could cause a flash fire or explosion due to electrostatic discharge. Containers of this material may be hazardous when empty since they retain product residues (dust, solids); observe all warnings and precautions listed for the product.


8. Exposure Controls/Personal Protection

Airborne Exposure Limits:
None established.

Ventilation System:
A system of local and/or general exhaust is recommended to keep employee exposures as low as possible. Local exhaust ventilation is generally preferred because it can control the emissions of the contaminant at its source, preventing dispersion of it into the general work area. Please refer to the ACGIH document, Industrial Ventilation, A Manual of Recommended Practices, most recent edition, for details.

Personal Respirators (NIOSH Approved):
For conditions of use where exposure to the dust or mist is apparent, a half-face dust/mist respirator may be worn. For emergencies or instances where the exposure levels are not known, use a full-face positive-pressure, air-supplied respirator. WARNING: Air-purifying respirators do not protect workers in oxygen-deficient atmospheres.

Skin Protection:
Wear protective gloves and clean body-covering clothing.

Eye Protection:
Use chemical safety goggles. Maintain eye wash fountain and quick-drench facilities in work area.


9. Physical and Chemical Properties

Appearance:
Dark red crystals.

Odor:
Odorless.

Solubility:
5 grams in 100 grams of water.

Specific Gravity:
No information found.

pH:
No information found.

% Volatiles by volume @ 21C (70F):
0

Boiling Point:
Not applicable.

Melting Point:
238 - 240C (460 - 464F)

Vapor Density (Air=1):
No information found.

Vapor Pressure (mm Hg):
No information found.

Evaporation Rate (BuAc=1):
No information found.


10. Stability and Reactivity

Stability:
Stable under ordinary conditions of use and storage.

Hazardous Decomposition Products:
Toxic gases and vapors may be released if involved in a fire.

Hazardous Polymerization:
Will not occur.

Incompatibilities:
Strong oxidizers.

Conditions to Avoid:
Heat, flames, ignition sources and incompatibles.


11. Toxicological Information


No LD50/LC50 information found relating to normal routes of occupational exposure. Investigated as a tumorigen and mutagen.

  --------\Cancer Lists\------------------------------------------------------
                                         ---NTP Carcinogen---
  Ingredient                             Known    Anticipated    IARC Category
  ------------------------------------   -----    -----------    -------------
  Ethidium Bromide (1239-45-8)            No          No            None


12. Ecological Information

Environmental Fate:


Environmental Toxicity:
No information found.


13. Disposal Considerations

Whatever cannot be saved for recovery or recycling should be managed in an appropriate and approved waste disposal facility. Processing, use or contamination of this product may change the waste management options. State and local disposal regulations may differ from federal disposal regulations. Dispose of container and unused contents in accordance with federal, state and local requirements.


14. Transport Information

Not regulated.


15. Regulatory Information


  --------\Chemical Inventory Status - Part 1\---------------------------------
  Ingredient                                       TSCA  EC   Japan  Australia
  -----------------------------------------------  ----  ---  -----  ---------
  Ethidium Bromide (1239-45-8)                    Exempt Yes   No       Yes                                       
 
  --------\Chemical Inventory Status - Part 2\---------------------------------
                                                          --Canada--
  Ingredient                                       Korea  DSL   NDSL  Phil.
  -----------------------------------------------  -----  ---   ----  -----
  Ethidium Bromide (1239-45-8)                      Yes   No    No     Yes                             
 
  --------\Federal, State & International Regulations - Part 1\----------------
                                             -SARA 302-    ------SARA 313------
  Ingredient                                 RQ    TPQ     List  Chemical Catg.
  -----------------------------------------  ---   -----   ----  --------------
  Ethidium Bromide (1239-45-8)               No    No      No         No
 
  --------\Federal, State & International Regulations - Part 2\----------------
                                                        -RCRA-    -TSCA-
  Ingredient                                 CERCLA     261.33     8(d) 
  -----------------------------------------  ------     ------    ------
  Ethidium Bromide (1239-45-8)               No         No         No                   
 
 
Chemical Weapons Convention:  No     TSCA 12(b):  No     CDTA:  No
SARA 311/312:  Acute: Yes      Chronic: No   Fire: No  Pressure: No
Reactivity: No          (Pure / Solid) 


Australian Hazchem Code: No information found.
Poison Schedule: No information found.

WHMIS:
This MSDS has been prepared according to the hazard criteria of the Controlled Products Regulations (CPR) and the MSDS contains all of the information required by the CPR.


16. Other Information

NFPA Ratings: Health: 1 Flammability: 1 Reactivity: 0

Label Hazard Warning:
WARNING! MAY BE HARMFUL IF SWALLOWED, INHALED OR ABSORBED THROUGH SKIN. MAY CAUSE IRRITATION TO SKIN, EYES, AND RESPIRATORY TRACT.

Label Precautions:
Avoid breathing dust. Avoid contact with eyes, skin and clothing. Keep container closed. Use with adequate ventilation. Wash thoroughly after handling. INTENDED FOR R & D USE ONLY. NOT ON THE TSCA INVENTORY.

Label First Aid:
If swallowed, induce vomiting immediately as directed by medical personnel. Never give anything by mouth to an unconscious person. If inhaled, remove to fresh air. Get medical attention for any breathing difficulty. In case of contact, immediately flush eyes or skin with plenty of water for at least 15 minutes. Get medical attention if irritation develops or persists.

Product Use:
Research and Development Use Only.

Revision Information:
Pure. New 16 section MSDS format, all sections have been revised.

Disclaimer:
************************************************************************************************ Mallinckrodt Baker, Inc. provides the information contained herein in good faith but makes no representation as to its comprehensiveness or accuracy. This document is intended only as a guide to the appropriate precautionary handling of the material by a properly trained person using this product. Individuals receiving the information must exercise their independent judgment in determining its appropriateness for a particular purpose. MALLINCKRODT BAKER, INC. MAKES NO REPRESENTATIONS OR WARRANTIES, EITHER EXPRESS OR IMPLIED, INCLUDING WITHOUT LIMITATION ANY WARRANTIES OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE WITH RESPECT TO THE INFORMATION SET FORTH HEREIN OR THE PRODUCT TO WHICH THE INFORMATION REFERS. ACCORDINGLY, MALLINCKRODT BAKER, INC. WILL NOT BE RESPONSIBLE FOR DAMAGES RESULTING FROM USE OF OR RELIANCE UPON THIS INFORMATION.
************************************************************************************************ Prepared by: Strategic Services Division
Phone Number: (314) 539-1600 (U.S.A.)